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Fully automated feedback controlled pressure polishing
- Make your own glass chips
- Optimise electrode shape for your experiment
- Fully automated multi-cycle pressure polishing
- Select your desired geometry by software
- nsert pulled patch electrode and start microforge
- Watch pipette shaping on screen
- Uses regular pulled borosilicate glass electrodes
- Gas flow cools tip opening during pressure polishing,
preserving the critical seal area
- Precise reproducible tip geometry on a micron
resolution
- Built-in machine vision CCD microscope
- Machine vision software controls process parameters
and tip geometry
- Tip recognition, positioning and shaping fully
automated
- Easily programmed controlling software
- Shaft and tip areas are blown in separate polishing
steps
- Measures and adjusts up to 8 diameters at chosen
distance from the tip
- Adapters for different capillary diameters available
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Example of two-step pressure
polishing cycle using the Feedback Microforge
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Intracellular access revolutionized
- Extremely fast internal perfusion of the pipette
- Intracellular solution exchange within <100 ms
- Rapid application of second messenger
- Rapid wash
- Quick cytoplasmic dialysis with dyes and chemicals
- Access time for perforated-patch is reduced to <10s
- 100% reliable perforation, perforated Rs <10 MOhm
Optimal passive properties
- Reduces space clamp problems
- Virtually eliminates shaft resistance and RC chain
- Enables precise and reproducible coating down
to the “remnant tip”
- Unprecedented RC characteristics
- Allows for perfect capacitance and Rs compensation
- Ideally suited for capacity measurements
- Ideally suited for single channel analysis
- Reduces run-down problems at low Rs values
- Precisely reproducible pipette properties
- Suited for PatchBox
- High success rates with any cell size
- Extremely fast extracellular compound addition
- Rapid ligand exposure (tau<50 ms)
- Quick and complete washout
- Compound volumes < 1 µl
- Low pipette capacitance (10 pF uncoated)
- Low and stable access resistance
- Dimensions (D x W x H):
(31.1 x 48.3 x 14.5) cm, (12.3 x 19.0 x 5.7) inches
- Weight: 12 kg / 25 lbs
- 90-240V AC
- Zeiss™ optics
- Standard pipette holder for 1.5 mm outer diameter. Adapters
for different diameters available.
- Accepts borosilicate and soft glass
- Example programs for thin-walled borosilicate capillaries included.
Other glass on request.
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